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microfluidic 96 96  (fluidigm)


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    fluidigm microfluidic 96 96
    Microfluidic 96 96, supplied by fluidigm, used in various techniques. Bioz Stars score: 96/100, based on 294 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microfluidic 96 96/product/fluidigm
    Average 96 stars, based on 294 article reviews
    microfluidic 96 96 - by Bioz Stars, 2026-03
    96/100 stars

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    Figure 8. Messenger RNA levels of annexins (ANX) and carbonic anhydrase 4 (CA4). (A) Anxa1, Anxa2 and Anxa8 mRNA levels. Following reverse transcription, mRNA level was quantified using the Biomark <t>microfluidic</t> system (BMK-M-96.96; Fluidigm); n=6. Relative quantification was normalized with eight housekeeping genes using GenNorm software. (B) Ca4 reverse transcription-PCR (100-bp DNA ladder). Messenger RNA level was assessed during the active growth phase of mineralization 10 h p.o. except for B at 18 h p.o.. Tibial bone, B; duodenum, D; kidney, K; Liver, L; Magnum, Ma; White isthmus, WI; Red isthmus, RI; Uterus, Ut; H2O, negative control; Std, size (bp) of DNA ladder (100- bp); n=2. Letters at the top indicate statistically significant differences in ANXs mRNA levels between the tissues (ANOVA-Tukey). The gene accession numbers and primers are compiled in Table S3. GraphPad Prism software was used for diagram representation
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    Figure 8. Messenger RNA levels of annexins (ANX) and carbonic anhydrase 4 (CA4). (A) Anxa1, Anxa2 and Anxa8 mRNA levels. Following reverse transcription, mRNA level was quantified using the Biomark microfluidic system (BMK-M-96.96; Fluidigm); n=6. Relative quantification was normalized with eight housekeeping genes using GenNorm software. (B) Ca4 reverse transcription-PCR (100-bp DNA ladder). Messenger RNA level was assessed during the active growth phase of mineralization 10 h p.o. except for B at 18 h p.o.. Tibial bone, B; duodenum, D; kidney, K; Liver, L; Magnum, Ma; White isthmus, WI; Red isthmus, RI; Uterus, Ut; H2O, negative control; Std, size (bp) of DNA ladder (100- bp); n=2. Letters at the top indicate statistically significant differences in ANXs mRNA levels between the tissues (ANOVA-Tukey). The gene accession numbers and primers are compiled in Table S3. GraphPad Prism software was used for diagram representation

    Journal: Journal of Biological Chemistry

    Article Title: Avian eggshell formation reveals a new paradigm for vertebrate mineralization via vesicular amorphous calcium carbonate

    doi: 10.1074/jbc.ra120.014542

    Figure Lengend Snippet: Figure 8. Messenger RNA levels of annexins (ANX) and carbonic anhydrase 4 (CA4). (A) Anxa1, Anxa2 and Anxa8 mRNA levels. Following reverse transcription, mRNA level was quantified using the Biomark microfluidic system (BMK-M-96.96; Fluidigm); n=6. Relative quantification was normalized with eight housekeeping genes using GenNorm software. (B) Ca4 reverse transcription-PCR (100-bp DNA ladder). Messenger RNA level was assessed during the active growth phase of mineralization 10 h p.o. except for B at 18 h p.o.. Tibial bone, B; duodenum, D; kidney, K; Liver, L; Magnum, Ma; White isthmus, WI; Red isthmus, RI; Uterus, Ut; H2O, negative control; Std, size (bp) of DNA ladder (100- bp); n=2. Letters at the top indicate statistically significant differences in ANXs mRNA levels between the tissues (ANOVA-Tukey). The gene accession numbers and primers are compiled in Table S3. GraphPad Prism software was used for diagram representation

    Article Snippet: Following reverse transcription, mRNA level was quantified using the Biomark microfluidic system (BMK-M-96.96; Fluidigm); n=6.

    Techniques: Reverse Transcription, Quantitative Proteomics, Software, Negative Control